基于SERS纳米探针的细胞内硝基还原酶检测

在缺氧的肿瘤细胞内, 硝基还原酶(NTR)通常过表达且其含量高低与缺氧程度呈正相关, 因此开发高选择性检测NTR的方法对早期肿瘤诊断至关重要. 本文通过修饰对硝基苯硫酚(p?NTP)到金纳米粒子(Au NPs)表面构建了一种表面增强拉曼散射(SERS)探针. 在缺氧条件下, 以还原型烟酰胺腺嘌呤二核苷酸(NADH)作为电子供体, NTR可催化还原芳香硝基为芳香胺, 导致纳米探针的SERS光谱发生变化, 从而实现NTR的高选择性检测, 检出限低至18 ng/mL. 该探针毒性低、 生物兼容性好, 可用于缺氧条件下A549细胞内的NTR分析, 为肿瘤细胞的缺氧现象评估提供了一种有效的策略.     

MULTIPLE SOLUTIONS TO A SECOND-ORDER m-POINT BOUNDARY VALUE PROBLEM

In this paper, we are concerned a class of second-order m-point boundary value problem. The existence results of at least three positive solutions are given by using a fixed-point theorem and imposing growth conditions on the nonlinear term, which depends on the first derivative.     

有限群的弱S-拟正规嵌入子群

群G的子群H称为在G中S-拟正规嵌入的,如果对于任意的素数p||H|,H的Sylow p-子群也是G的某个S-拟正规子群的Sylow p-子群.称群G的子群H在G中弱S-拟正规嵌入,如果存在群G的正规子群T,使得HT■G且H∩T在G中是S-拟正规嵌入的.研究了弱S-拟正规嵌入子群的性质,给出了某些群类的新的特征,并推广了一些已知的结论.     

Bilinear approach for a symmetry constraint of the modified KdV equation

The paper investigates the mKdV equation with the potential under symmetry constraint through bilinear approach, i.e., Hirota method and Wronskian technique. We show that the potential can be a summation of squares of wave functions and these wave functions can precisely be described as Wronskians.     

子群的S-条件置换性对有限群结构的影响

Let G be a finite group. Fix a prime divisor p of IGI and a Sylow p-subgroup P of G, let d be the smallest generator number of P and Ma（P） denote a family of maximal subgroups P1, P2 , Pd of P satisfying ∩^di=1 Pi = Ф（P）, the Frattini subgroup of P. In this paper, we shall investigate the influence of s-conditional permutability of the members of some fixed .Md（P） on the structure of finite groups. Some new results are obtained and some known results are generalized.     

Surface profile inspection of a moving object by using dual-frequency Fourier transform profilometry

The 2π phase ambiguity caused by surface isolations and large height step can be solved by dual-frequency projection grating profilometry. However, in the Fourier transform profilometry (FTP) of a moving object, only one single deformed fringe pattern can be obtained. In order to introduce the dual-frequency technique into the FTP of moving object, a novel experimental system is designed to capture two fringe patterns with different frequency at the same time. A grating structure comprising two regions with different frequencies is projected upon the surface of the detected object. Two line-scan CCD cameras are used to capture the surface images encoded by the two kinds of patterns, respectively. By getting the corresponding image intensity at the same point of the object surface in the two acquired images, the dual-frequency technique is applied to extract the real phase without phase ambiguity. The surface profile of a specimen with a large height step is measured to prove the feasibility of the proposed method. The experimental results show that the proposed method can solve the 2π phase ambiguity problem successfully in the surface profile inspection of a moving object.     

The fluctuating phase error analysis in the digital grating phase-shifting profilometry

The nonlinear response of the experimental system and the saturation of fringe patterns can induce the fluctuating phase error in the projection grating phase-shifting profilometry. Two major factors of the fluctuating phase error are discussed by simulation. The fluctuating phase error caused by the nonlinear response of the system is four times the frequency of the fringe pattern when the conventional four-frame phase extracting algorithm is used. However, such error can be decreased by five-frame algorithm. On the other hand, the fluctuating phase error caused by the fringe saturation is five times the frequency of the fringe pattern by using conventional five-frame phase extracting algorithm. A novel phase recovering algorithm is used to decrease the phase error caused by the saturation. Furthermore, the applicability range of the proposed phase recovering algorithm is analyzed by simulation and experiments with different saturation degree of the fringe pattern and nonlinearity of the measurement system.     

BIG6模式下利用废旧设备开发光电实验

废旧设备作为高校的一类固定资产,如何处理好它,尽量发挥其残值,是高校设备管理的一个课题.相当多的废旧设备属于光电类器件,包含各自的光电工作原理,如激光打印机、光电鼠标、光驱等,利用此类废旧设备开发出对应的光电实验,可以作为光信息科学与技术专业的补充实验.开发过程基于BIG6信息问题解决模式,强调师生互动.提高学生的动手...     

New formal (3+3) cycloaddition of enaminones for forming tetracyclic indolo[2,3-b]quinolines under microwave irradiation

Concise and efficient base-promoted domino formal (3+3) cycloaddition has been established for the formation of tetracyclic indolo[2,3-b]quinoline derivatives under microwave heating. The present methodology shows attractive properties, such as the maximum efficiency of a process, short reaction periods, and operational simplicity, and it can avoid time-consuming and costly syntheses, tedious work-up and isolation of intermediate. This chemistry provides a facile and promising synthetic strategy to construction of tetracyclic indolo[2,3-b]quinoline skeleton.     

Optimised synthesis of diamino-triazinylmethyl benzoates as inhibitors of Rad6B ubiquitin conjugating enzyme

Recently, we have identified the first inhibitors of Rad6B, an E2 enzyme essential for post-replication DNA repair and a potential new drug target for the treatment of breast cancer. We report two newly optimised synthetic routes to our [4-amino-6-(phenylamino)-1,3,5-triazin-2-yl]methyl 4-nitrobenzoate target compounds TZ8 and TZ9 with general applicability for further structure–activity relationship studies around this pharmacophore. The key step involved the condensation/cyclisation between phenylbiguanide and either ethyl bromoacetate or dimethyloxalate in good yield.